Premium
Highly sensitive immunohistological study for detection of estrogen receptor in human breast cancer
Author(s) -
Sumiyoshi Yoshiaki,
Shirakusa Takayuki,
Yamashita Yuichi,
Maekawa Takafumi,
Sakai Toshimi,
Kikuchi Masahiro
Publication year - 2001
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/1096-9098(200101)76:1<13::aid-jso1003>3.0.co;2-m
Subject(s) - estrogen receptor , immunohistochemistry , medicine , estrogen , breast cancer , receptor , immunoassay , pathology , antigen , cancer , cancer research , antibody , immunology
Background and Objectives Immunohistological methods for the detection of estrogen receptor in human breast cancer using formalin‐fixed, paraffin‐embedded tissue not only have the advantages of being cheaper and requiring less tissue but also have several theoretical advantages. For example, such methods enable direct histological visualization of the assessed tissue and thereby reduce sampling error. Traditional immunohistological methods (traditional LSAB), however, show false‐negative reactions more often than the enzyme‐binding immunoassay method (EIA). Methods After determining 25 estrogen receptor‐positive cases and 50 estrogen receptor‐negative cases by EIA, we analyzed these same cases using a traditional LSAB method and a new highly sensitive immunohistological method for assaying formalin‐fixed, paraffin‐embedded tissue. Results Using this new method, we detected 10 instances of positive estrogen receptors among the 50 EIA receptor‐negative cases. The 25 EIA‐positive cases were positive for estrogen receptor antigen except 1case using the new method. Conclusions This new method not only has the advantage of use in paraffin‐embedded tissue but also is more sensitive than the EIA. We recommend this new method as a choice to decrease false‐negatives. J. Surg. Oncol. 2001;76:13–18. © 2001 Wiley‐Liss, Inc.