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Characterization of human group C rotavirus in Argentina
Author(s) -
Castello Alejandro A.,
Argüelles Marcelo H.,
Villegas Guillermo A.,
López Nora,
Ghiringhelli Daniel P.,
Semorile Liliana,
Glikmann Graciela
Publication year - 2000
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/1096-9071(200010)62:2<199::aid-jmv11>3.0.co;2-x
Subject(s) - rotavirus , virology , diarrhea , homology (biology) , reoviridae , biology , group a , nucleic acid sequence , virus , gene , genetics , medicine
A survey was conducted for identification of human group C rotaviruses in stool specimens taken from children suffering diarrhea in suburban Buenos Aires regions. Among 90 true negative group A samples as defined by ELISA, RT‐PCR and PAGE, five were positive by group C specific RT‐PCR (VP7 and VP6 genes) and three of these samples exhibited the characteristic 4‐3‐2‐2 dsRNA pattern of group C rotavirus. These results were further confirmed by electron microscopy and by ELISA for detection of group C VP6 specific antigens. Sequence analysis of the VP7 gene from one of these isolates revealed a 97.3–98.6% nucleotide identity and up to 99.1% protein homology with human group C rotavirus strains found scattered throughout the last ten years in other countries. Conversely, similar analysis performed with porcine strains showed a much lower homology degree both at the nucleotide (75.5% nucleotide identity) and amino acid level (85.5% protein homology). Detection of group C rotavirus in children with acute diarrhea in Argentina extends the identification range of this agent in the region and is consistent with previous reported data that demonstrate a global distribution of this virus. J. Med. Virol. 62:199–207, 2000. © 2000 Wiley‐Liss, Inc.