Premium
Molecular epidemiology of astroviruses in Japan from 1995 to 1998 by reverse transcription‐polymerase chain reaction with serotype‐specific primers (1 to 8)
Author(s) -
Sakamoto Tatsunori,
Negishi Hideo,
Wang QiuHong,
Akihara Shiho,
Kim Bosu,
Nishimura Shuichi,
Kaneshi Kunio,
Nakaya Shigekazu,
Ueda Yuichi,
Sugita Kumiko,
Motohiro Takashi,
Nishimura Tadafumi,
Ushijima Hiroshi
Publication year - 2000
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/1096-9071(200007)61:3<326::aid-jmv7>3.0.co;2-w
Subject(s) - serotype , astrovirus , virology , polymerase chain reaction , biology , reverse transcription polymerase chain reaction , reverse transcriptase , primer (cosmetics) , molecular epidemiology , virus , genotype , genetics , gene , rotavirus , chemistry , messenger rna , organic chemistry
In addition to the serotype‐specific primers described previously (1 to 7), a new serotype 8‐specific primer has been designed, allowing detection of all astrovirus serotypes. A total of 1,382 diarrheal stool samples in 5 regions in Japan were examined by reverse transcription‐polymerase chain reaction (RT‐PCR). The incidence of astrovirus infection in all 5 regions was 5.9% (82 of 1,382 samples) and infection occurred mainly from November to April. Serotypes 1, 3, and 4 were detected in 66, 14, and 2 of the 82 positive samples, respectively. None of the other serotypes was detected. The highest detection rate was from 0 to 1 year old, 39.0%, and the next highest was from 1 to 2 years old, 34.1%. The primers provide a useful approach for study of the epidemiology of astroviruses. J. Med. Virol. 61:326–331, 2000. © 2000 Wiley‐Liss, Inc.