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A de novo Philadelphia chromosome‐positive acute mixed‐lineage leukemia with both major and minor BCR/ABL mRNA transcripts
Author(s) -
Tarumoto Takahisa,
Imagawa Shigehiko,
Ohmine Ken,
Mano Atsushi,
Nagai Tadashi,
Takatoku Masaaki,
Muroi Kazuo,
Hatake Kiyohiko,
Ozawa Keiya
Publication year - 2000
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/1096-8652(200009)65:1<72::aid-ajh13>3.0.co;2-r
Subject(s) - biology , microbiology and biotechnology , abl , myeloid leukemia , messenger rna , reverse transcriptase , polymerase chain reaction , southern blot , lineage (genetic) , breakpoint cluster region , philadelphia chromosome , gene , leukemia , northern blot , immunoglobulin heavy chain , cancer research , genetics , chromosomal translocation , tyrosine kinase , receptor
A patient with a Philadelphia chromosome (Ph)‐positive acute mixed‐lineage leukemia (AMLL) expressing both major and minor BCR/ABL mRNA transcripts is described. Phenotypic analysis of the leukemic blasts revealed positivity for both myeloid and B‐cell lineages. Southern blot analysis showed a rearrangement of the immunoglobulin heavy chain (IgH) gene. Reverse transcriptase polymerase chain reaction (RT‐PCR) analysis showed the expression of both major and minor BCR/ABL mRNA transcripts. To our knowledge, this is the first report of AMLL expressing both major and minor BCR/ABL mRNA transcripts and rearrangement of the IgH gene. Am. J. Hematol. 65:72–74, 2000. © 2000 Wiley‐Liss, Inc.