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Vitamin E reduces platelet adhesion to human endothelial cells in vitro
Author(s) -
Szuwart T.,
Brzoska T.,
Luger T.A.,
Filler T.,
Peuker E.,
Dierichs R.
Publication year - 2000
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/1096-8652(200009)65:1<1::aid-ajh1>3.0.co;2-8
Subject(s) - platelet , adhesion , chemistry , thrombin , vitamin e , endothelial stem cell , platelet rich plasma , tocopherol , in vitro , alpha tocopherol , vitamin , cell adhesion , platelet adhesiveness , biochemistry , in vivo , medicine , cell , biology , antioxidant , platelet aggregation , microbiology and biotechnology , organic chemistry
Although it has been reported that vitamin E (α‐tocopherol) can reduce platelet adhesiveness and aggregation in vivo, the mechanism is still unknown. Therefore, the aim of the present study was to determine whether incubations of platelet‐rich plasma (PRP) with vitamin E influence platelet adhesion to cultured endothelial cells. To exclude blood plasma involvement, also washed platelets were pretreated with α‐tocopherol. Vitamin E (0.5–1.0 mM) was added to PRP or washed platelets. Endothelial cells in monolayer were incubated with thrombin‐activated platelets (1 or 2 U/ml). After 1 hr of incubation, non‐adhered platelets were removed and counted. Treating of PRP with α‐tocopherol inhibited platelet adhesion to endothelial cell monolayer. This effect was dose dependent on concentrations of α‐tocopherol and thrombin. In our experiments PRP was treated with α‐tocopherol and endothelial cell monolayer was used as test surface. These findings agree with previous observations on the adhesivity of platelets to synthetic surfaces after dietary vitamin E in healthy volunteers. When washed platelets were incubated with α‐tocopherol, no significant reduction of adhesion was detectable. As preincubation of washed platelets with α‐tocopherol does not inhibit platelet adhesion, it may be supposed that the effect of vitamin E does not occur in a directly cellular mechanism. The data suggest that α‐tocopherol may reduce platelet adhesiveness probably after incorporation by plasma lipoproteins. Am. J. Hematol. 65:1–4, 2000. © 2000 Wiley‐Liss, Inc.