Open AccessApplication of Dynamic Light Scattering in Protein Crystallization
Author(s) -
Proteau Ariane,
Shi Rong,
Cygler Miroslaw
Publication year - 2010
Publication title -
current protocols in protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.409
H-Index - 32
eISSN - 1934-3663
pISSN - 1934-3655
DOI - 10.1002/0471140864.ps1710s61
Subject(s) - crystallization , homogeneity (statistics) , dynamic light scattering , macromolecule , protein crystallization , scattering , diffraction , light scattering , crystallography , chemistry , chemical physics , materials science , biological system , biophysics , chemical engineering , nanotechnology , optics , physics , computer science , biochemistry , biology , nanoparticle , organic chemistry , engineering , machine learning
Success in determining the three‐dimensional structure of a macromolecule by X‐ray diffraction methods depends critically on the ability to obtain well ordered crystals of the macromolecule in question. Predisposition to crystallization correlates with the homogeneity of the molecules in solution. Dynamic light scattering (DLS) is particularly well suited for evaluating protein homogeneity under multiple conditions and at concentrations commensurate with crystallization conditions. This unit presents a typical protocol for DLS measurements of a protein sample, and describes approaches to improve protein homogeneity in solution. Curr. Protoc. Protein Sci . 61:17.10.1‐17.10.9. © 2010 by John Wiley & Sons, Inc.
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