Premium
Deletion of Dad1 in mice induces an apoptosis‐associated embryonic death
Author(s) -
Brewster Jay L.,
Martin Stacey L.,
Toms Jennifer,
Goss Dee,
Wang Kia,
Zachrone Keith,
Davis Angela,
Carlson George,
Hood Leroy,
Coffin J. Douglas
Publication year - 2000
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/(sici)1526-968x(200004)26:4<271::aid-gene90>3.0.co;2-e
Subject(s) - biology , apoptosis , gene targeting , embryo , blastocyst , microbiology and biotechnology , offspring , embryogenesis , andrology , gene , genetics , medicine , pregnancy
Summary: Dad1 is a putative anti‐apoptosis gene identified in several distantly related organisms. Expression of Dad 1 in transfected cells inhibits apoptosis in vitro. To determine whether Dad 1 has a similar function in vivo, we used gene targeting to delete Dad 1. Heterozygous adult mice (+/−) show no obvious phenotype or abnormalities, but genotype analysis of over 100 offspring from heterozygous matings detected no weanling, homozygous Dad 1 null (−/−) mice. Subsequent analysis of embryos from heterozygous matings detected Dad 1 null (−/−) embryos at E3.5 but no later, suggesting Dad 1 is required for development beyond the late blastocyst stage. Increased levels of apoptosis were observed in cultured embryos lacking a functional copy of the gene, consistent with an anti‐apoptotic role for Dad 1. genesis 26:271–278, 2000. © 2000 Wiley‐Liss, Inc.