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psb A Mutation (valine 219 to isoleucine) in Poa annua resistant to metribuzin and diuron
Author(s) -
Mengistu Lemma W,
MuellerWarrant George W,
Liston Aaron,
Barker Reed E
Publication year - 2000
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/(sici)1526-4998(200003)56:3<209::aid-ps117>3.0.co;2-8
Subject(s) - metribuzin , biology , photosystem ii , acetolactate synthase , chlamydomonas , genetics , mutagenesis , mutation , valine , gene , botany , mutant , amino acid , photosynthesis , weed control , agronomy
The herbicide‐binding region of the chloroplast psb A gene from a total of 20 biotypes of Poa annua L resistant and susceptible to metribuzin and diuron was selectively amplified using PCR. Sequence analysis of the fragment from six herbicide‐resistant biotypes of P annua exhibited a substitution from valine to isoleucine at position 219 of the D1 protein encoded by the psb A gene. This is the same mutation as reported for Chlamydomonas and Synechococcus through site‐directed mutagenesis and in cell cultures of Chenopodium rubrum L. To our knowledge this is the first report of a higher plant exhibiting resistance in the field to photosystem II inhibitors due to a psb A mutation other than at position 264. The existence of additional biotypes of P annua resistant to diuron or metribuzin but lacking mutation in the herbicide‐binding region indicates that resistance to these herbicides can also be attained by other mechanisms. © 2000 Society of Chemical Industry