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Aminopyrazine analogues as chemiluminescence derivatization reagents for pyruvic acid
Author(s) -
Sakata Miyuki,
Sakata Noriaki,
Ohba Yoshihito,
Zaitsu Kiyoshi
Publication year - 1999
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/(sici)1522-7243(199911/12)14:6<365::aid-bio564>3.0.co;2-4
Subject(s) - pyrazine , chemistry , chemiluminescence , pyruvic acid , reagent , derivatization , hydrogen peroxide , dimethylformamide , amino acid , organic chemistry , medicinal chemistry , high performance liquid chromatography , biochemistry , solvent
Aminopyrazine analogues were studied as sensitive and selective chemiluminescence derivatization reagents for pyruvic acid. These analogues reacted with pyruvic acid under acidic conditions at 100°C to produce Cypridina luciferin derivatives, which exhibit chemiluminescence by reaction with hydrogen peroxide in the presence of potassium t ‐butoxide in dimethylformamide. Of the four aminopyrazine analogues (2‐amino‐5‐phenylpyrazine, 2‐amino‐5‐(4‐hydroxyphenyl)pyrazine, 2‐amino‐5‐(3,4,5‐trimethoxyphenyl)pyrazine, and 2‐aminoquinoxaline), in the present test 2‐amino‐5‐(3,4,5‐trimethoxyphenyl) pyrazine was the most sensitive for pyruvic acid, and the chemiluminescence intensity was about four times higher than that obtained with aminopyrazine. Copyright © 1999 John Wiley & Sons, Ltd.