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Applicability of chemiluminescence to assess the degree of operative stress in patients undergoing spinal surgery
Author(s) -
Kowatari Kenji,
Suzuki Katsuhiko,
Kudo Satoru,
Yamada Mutsuo,
Liu Qiang,
Danjo Kazuma,
Umeda Takashi,
Nakaji Shigeyuki,
Sugawara Kazuo
Publication year - 1999
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/(sici)1522-7243(199911/12)14:6<331::aid-bio556>3.0.co;2-e
Subject(s) - leukocytosis , zymosan , medicine , granulocytosis , surgical stress , granulocyte , tumor necrosis factor alpha , chemiluminescence , morning , interleukin 6 , immunology , anesthesia , surgery , endocrinology , gastroenterology , cytokine , chemistry , biochemistry , organic chemistry , in vitro
This study examined the applicability of luminol‐dependent chemiluminescence (CL) response of neutrophils to assess the degree of stress of spinal surgery by measuring the capacity of circulating neutrophils to produce reactive oxygen species and the levels of serum cytokines: interleukin(IL)‐1β, IL‐6, IL‐8, tumour necrosis factor (TNF)‐α and granulocyte colony stimulating factor (G‐CSF). Ten male patients underwent spinal surgery. Peripheral blood samples, collected before and after the operation and the next morning, were used for measuring the CL response of neutrophils stimulated with opsonized zymosan and measuring the levels of serum cytokines. The operative stress induced leukocytosis, particularly granulocytosis, and increased serum IL‐6 and G‐CSF significantly. However, there was no significant change in the luminol‐dependent CL response of neutrophils or the levels of serum IL‐1β, IL‐8 and TNF‐α throughout the experimental period. These results suggest that, at least in the early postoperative period, operative stress does not prime the circulating neutrophils, and thus the CL response of neutrophils is not appropriate to assess the degree of stress of spinal surgery. Copyright © 1999 John Wiley & Sons, Ltd.