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Effect of decaglycerol mono‐oleate on chemiluminescence of human neutrophils
Author(s) -
Liu Qiang,
Suzuki Katsuhiko,
Kudo Satoru,
Yamada Mutsuo,
Kowatari Kenji,
Umeda Takashi,
Nakaji Shigeyuki,
Sugawara Kazuo
Publication year - 1999
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/(sici)1522-7243(199911/12)14:6<327::aid-bio571>3.0.co;2-a
Subject(s) - chemiluminescence , luminol , zymosan , chemistry , opsonin , reactive oxygen species , in vitro , phorbol , respiratory burst , biochemistry , chromatography , enzyme , protein kinase c
Decaglycerol mono‐oleate (DGMO), a type of food additive (a polyglycerol ester of fatty acids), was evaluated for its in vitro effect on reactive oxygen species (ROS) generation by human neutrophils using a luminol‐dependent chemiluminescence assay. Neutrophils were isolated by the Histopaque‐1077/1119 double density gradient technique. Opsonized zymosan (OZ) and phorbol 12‐myristate 13‐acetate (PMA) were employed as artificial stimuli. DGMO inhibited the chemiluminescence response of OZ‐stimulated neutrophils by 58% ( p < 0.01) at a concentration of 0.5 mg/mL. DGMO inhibited the PMA‐induced chemiluminescence by 40% ( p < 0.01) at a concentration of 0.01 mg/mL. The inhibition phenomena could not be attributed to light absorption by DGMO alone, because the percentage chemiluminescence in the presence of DGMO is lower than the percentage transmittance of DGMO at 425 nm. These results suggest that DGMO downregulates ROS generation by human neutrophils. Copyright © 1999 John Wiley & Sons, Ltd.