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Low‐level chemiluminescence of N ‐β‐alanyl‐ L ‐histidine ( L ‐carnosine)
Author(s) -
Achyuthan Komandoor E.
Publication year - 1999
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/(sici)1522-7243(199909/10)14:5<245::aid-bio541>3.0.co;2-2
Subject(s) - carnosine , anserine , chemistry , chemiluminescence , histidine , stereochemistry , chromatography , nuclear chemistry , enzyme , biochemistry
Oxidized N ‐β‐Ala‐ L ‐His ( L ‐carnosine) emitted low‐level CL. The CL specificity was shown by experiments with L ‐carnosine from six separate vendors, several L ‐carnosine‐like compounds, and nine different oxidizers. Purity of L ‐carnosine samples was analysed by RP–HPLC–MS, 1 H‐NMR, MALDI–TOF–MS and ESI–MS. L ‐Carnosine CL magnitude varied with source; consequently, detection sensitivity was 5–100 nmol. CL of L ‐anserine ( N ‐β‐Ala‐1‐methyl‐ L ‐His) was equal to or less than L ‐carnosine, depending upon oxidizer. H 5 IO 6 (2 mmol/L) in 11 mmol/L NaOH or 20 mmol/L K 3 Fe(CN) 6 + 10 mmol/L H 2 O 2 in 100 mmol/L NaOH were oxidizers of choice. Scavengers of · OH − radical quenched CL. Kinetic studies revealed a bi‐phasic CL comprising a short‐lived (<1 s) ‘flash’ and then prolonged (∼2000 s) ‘glow’. A structural basis and mechanism of L ‐carnosine CL are discussed. L ‐Carnosine CL could be useful for monitoring its level in biological samples. Copyright © 1999 John Wiley & Sons, Ltd.