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Practical enzymology course based on bioluminescence
Author(s) -
Kratasyuk V. A.,
Kudinova I. Y.
Publication year - 1999
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/(sici)1522-7243(199907/08)14:4<189::aid-bio527>3.0.co;2-e
Subject(s) - bioluminescence , enzyme , chemistry , substrate (aquarium) , biochemistry , biology , ecology
We describe our experience with laboratory courses in enzymology based on the phenomenon of bioluminescence. The soluble and immobilized enzymes of luminous bacteria are used and the practical enzymological course consists of four main courses: (1) training in measuring the activities of soluble and immobilized enzymes; (2) the investigation of kinetic characteristics (kinetic constants) and enzyme–substrate and enzyme–inhibitor interactions in the bacterial bioluminescent reaction; (3) The testing of physico‐chemical characteristics of enzymes (pH, temperature, ion strength, etc.); (4) the effect of inhibitors on enzymes. Training is possible in groups of about ten persons. Our practice work has been introduced in the biological, pedagogical and physical departments of Krasnoyarsk State University. Students of the pedagogical department have created a popular and interesting series of laboratory works for high school children aged 14–17 years. Copyright © 1999 John Wiley & Sons, Ltd.