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Inhibition of luminol and lucigenin chemiluminescence by reducing organic compounds
Author(s) -
Cui Hua,
Meng Rong,
Jiang Haiyan,
Sun Yugang,
Lin Xiangqin
Publication year - 1999
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/(sici)1522-7243(199905/06)14:3<175::aid-bio540>3.0.co;2-k
Subject(s) - lucigenin , chemiluminescence , luminol , chemistry , photochemistry , reducing agent , redox , inorganic chemistry , organic chemistry , enzyme , superoxide
The effect of 22 reducing organic compounds on the chemiluminescent intensities and spectra of luminol–H 2 O 2 –Co 2+ and lucigenin–H 2 O 2 –Co 2+ reactions were examined. Many of the reductants inhibit the two chemiluminescent systems to different extents in the presence and absence of Co 2+ . The magnitude of inhibition by reducing organic compounds is related to their molecular structure and reducing properties. The mechanism of chemiluminescent inhibition by reducing organic compounds is probably due to the reaction between reductants and radical intermediates of chemiluminescent reaction. Based on the inhibition of luminol and lucigenin chemiluminescence by reducing organic compounds, the possibility of analytical application was explored. Some reductants are detectable at picomolar levels. Copyright © 1999 John Wiley & Sons, Ltd.