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Chemiluminescent detection of catecholamines by generation of hydrogen peroxide with imidazole
Author(s) -
Nozaki Osamu,
Iwaeda Toshinao,
Moriyama Hiroyuki,
Kato Yoshio
Publication year - 1999
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/(sici)1522-7243(199905/06)14:3<123::aid-bio525>3.0.co;2-i
Subject(s) - chemiluminescence , peroxyoxalate , luminol , hydrogen peroxide , chemistry , horseradish peroxidase , peroxide , pyrogallol , detection limit , imidazole , photochemistry , nuclear chemistry , chromatography , organic chemistry , enzyme
A novel detection method for catecholamines using imidazole was investigated using a chemiluminescence coupled flow injection system. Imidazole catalysed decomposition of catecholamines to generate hydrogen peroxide, then the hydrogen peroxide was detected by chemiluminescence. The optimal condition for generation of hydrogen peroxide from a catecholamine was to incubate the catecholamines (53 pmol) in an imidazole solution (50 mmol/L, pH 9.0, 1.0 mL) at 60°C for 30 min. Peroxidewas detected by peroxyoxalate chemiluminescence, and the rank order of the light emission intensities was as follows; dopamine (100%) >epinephrine (78%) > L ‐DOPA (62%) >norepinephrine (58%) >deoxyepinephrine (51%) >isoproterenol (43%) >dihydroxybenzylamine (25%). The light intensities of the reaction mixtures (corresponding to 1.06 pmol catecholamines) varied depending on the chemiluminescence (CL) detection reaction, and the rank order of the light intensity was as follows; luminol CL catalysed with horseradish peroxidase (HRP) (371%) >peroxyoxalate chemiluminescence (100%) >luminol CL catalysed with ferrycianide (62%) >lucigenin CL (15%) >pyrogallol CL (0.8%) >purpurogallin CL (0.4%) >luminol CL (0.3%). The luminol CL reaction catalysed by HRP is recommended for the detection of peroxide in this method for catecholamines. Copyright © 1999 John Wiley & Sons, Ltd.