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Enhancement of aminophthalhydrazides chemiluminescence by N ‐ β ‐alanyl‐ L ‐histidine ( L ‐carnosine)
Author(s) -
Achyuthan Komandoor E.
Publication year - 1999
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/(sici)1522-7243(199903/04)14:2<75::aid-bio516>3.0.co;2-u
Subject(s) - chemiluminescence , carnosine , luminol , chemistry , chromatography , light emission , biochemistry , materials science , optoelectronics
L ‐Carnosine was found to be a potent enhancer of chemiluminescence from luminol, isoluminol, or N ‐aminobutyl‐ N ‐ethyl‐isoluminol oxidized by H 5 IO 6 (2 mmol/L in 11 mmol/L NaOH) or 20 mmol/L K 3 Fe(CN) 6 + 10 mmol/L H 2 O 2 in 100 mmol/L NaOH. Reproducible chemiluminescence enhancement was obtained with luminol and L ‐carnosine, each from six different vendors, isoluminol from two different vendors, and two different oxidizers. The magnitude of enhancement depended upon the source and concentration of L ‐carnosine, type and concentration of aminophthalhydrazide and choice and concentration of oxidizer. Excellent signal/noise and fold‐enhancement were obtained for all three aminophthalhydrazides by including 10 mmol/L L ‐carnosine during K 3 Fe(CN) 6 + H 2 O 2 oxidation. L ‐Carnosine increased the sensitivity of detection of the three aminophthalhydrazides by 25–100‐fold. A new combination of K 3 Fe(CN) 6 + H 2 O 2 concentration resulted in 20–30‐fold more light emission from N ‐aminobutyl‐ N ‐ethyl‐isoluminol in the presence of 10 mmol/L L‐carnosine compared to chemiluminescence generated by two previously published concentrations of this oxidizer. L ‐Carnosine could further improve the sensitivity of chemiluminescent assays for factor XIIIa and transglutaminase. Copyright © 1999 John Wiley & Sons, Ltd.