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Proteome analysis of Oncorhynchus species during embryogenesis
Author(s) -
Kanaya Shigehiko,
Ujiie Yoshihumi,
Hasegawa Katumi,
Sato Takehiro,
Imada Hayato,
Kinouchi Makoto,
Kudo Yoshihiro,
Ogata Tateaki,
Ohya Hiroaki,
Kamada Hitoshi,
Itamoto Kenji,
Katsura Kazuhiko
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000501)21:9<1907::aid-elps1907>3.0.co;2-e
Subject(s) - rainbow trout , vitellogenin , biology , proteome , trout , oncorhynchus , embryo , genetics , fish <actinopterygii> , fishery
To understand the molecular mechanisms underlying normal and abnormal development of two salmonids, masu salmon ( Oncorhynchus masou ) and rainbow trout ( O. mykiss ), we used two‐dimensional (2‐D) electrophoresis to construct a series of 2‐D maps during the embryonic period. We identified all visible protein spots on the 2‐D map by assigning numbers for masu salmon and rainbow trout, and we determined N ‐terminal sequences of proteins for one hundred of the spots, that appear at very high concentrations in the whole embryos of masu salmon and rainbow trout. We also characterized embryonic stages according to the periods of appearance of spots. Most of the N ‐terminal sequences were identical or at least highly similar to partial sequences reported for vitellogenin (Vtg) of O. mykiss . A potential proteolytic processing of Vtg for rainbow trout is discussed in relation to the time of appearance and relative position of Vtg fragments within the complete protein sequence.