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Protein synthesis in Escherichia coli at 4°C
Author(s) -
Perrot Frédérique,
Hébraud Michel,
Junter GuyAlain,
Jouenne Thierry
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000501)21:8<1625::aid-elps1625>3.0.co;2-4
Subject(s) - escherichia coli , mesophile , bacteria , incubation , chemistry , gel electrophoresis , protein biosynthesis , biochemistry , electrophoresis , biology , microbiology and biotechnology , gene , genetics
Two‐dimensional electrophoresis technology was used to investigate protein synthesis by the mesophilic bacterium Escherichia coli at low temperature. It was confirmed that protein synthesis in E. coli decreased strongly after a temperature downshift from 37 to 4°C. After incubation for 150 min at 4°C, however, the number of synthesized proteins represented 60% of the overall polypeptide number observed at 37°C. Furthermore, the analysis of autoradiograms revealed the overexpression of 69 proteins by shocked bacteria, showing that the number of cold‐induced proteins has been significantly underestimated so far.