Premium
Preparation and characterization of calixarene‐coated capillaries for capillary electrophoresis
Author(s) -
Wang Zhixin,
Chen Yi,
Yuan Hushan,
Huang Zhitang,
Liu Guoquan
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000501)21:8<1620::aid-elps1620>3.0.co;2-y
Subject(s) - calixarene , capillary electrophoresis , chemistry , coating , capillary action , selectivity , chromatography , electrophoresis , absorption (acoustics) , organic chemistry , molecule , materials science , catalysis , composite material
Preparation and characterization of calixarene‐coated capillaries for capillary electrophoresis (CE) were exemplified with p ‐allylcalix[4]arene ( p ‐ACX 4 ) which was immobilized to the fused silica surface using γ‐methacryloxypropyl‐trimethoxysilane (γ‐MAPS) as linking agent. Successful p ‐ACX 4 coating was suggested by the greatly decreased electroosmotic flow (EOF), due to the introduction of phenolic hydroxyl groups on the inner surface of the capillaries. A slight slope of EOF versus pH at pH <8 would help make the separation reproducible. The coated columns also featured a low ultraviolet (UV) absorption background and long lifetime (> 6 months at 4