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Monitoring myoglobin by capillary zone electrophoresis with end‐column amperometric detection
Author(s) -
Jin Wenrui,
Dong Qian,
Yu Daiqing,
Ye Xiaoyin
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000501)21:8<1535::aid-elps1535>3.0.co;2-8
Subject(s) - myoglobin , capillary electrophoresis , amperometry , chromatography , capillary action , chemistry , capillary electrochromatography , electrophoresis , analytical chemistry (journal) , electrode , materials science , electrochemistry , organic chemistry , composite material
Capillary zone electrophoresis was employed for the determination of myoglobin in human urine using end‐column amperometric detection with a carbon fiber microelectrode at a constant potential of 1.80 V vs. saturated calomel electrode (SCF). The optimum conditions of separation and detection are: 3.73 × 10 −4 mol/L sodium diethyl malonyl urea (barbitone sodium), 1.34 × 10 −4 mol/L HCl for the buffer solution, 20 kV for separation voltage, 5 kV and 5 s for injection voltage and injection time, respectively. The limit of detection is 4.4 × 10 −8 mol/L or 84 amole signal to noise (S/N = 2). The relative standard deviation is 2.9% for the migration time and 2.5% for the electrophoretic peak current. The method can be used for the determination of myoglobin in human urine. The samples can be directly injected and need no pretreatment. The method is also rapid, less than 2 min, and has a recovery rate of 94—106%.