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A new absorbed coating for DNA fragment analysis by capillary electrophoresis
Author(s) -
Chiari Marcella,
Cretich Marina,
Horvath Judit
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000501)21:8<1521::aid-elps1521>3.0.co;2-y
Subject(s) - coating , adsorption , biopolymer , capillary electrophoresis , capillary action , polymer , chemical engineering , electrophoresis , matrix (chemical analysis) , chemistry , materials science , ether , chromatography , nanotechnology , organic chemistry , composite material , engineering
A fully automated coating procedure was devised based on adsorption of a new polymer, poly(dimethylacrylamide‐ co ‐allyl glycidyl ether) onto the capillary surface. The whole procedure takes less than 30 min and does not require the use of organic solvents, viscous solutions, or elevated temperature. The coating is stable even under harsh conditions such as alkaline pH, elevated temperature, and denaturant conditions that destroy most other presently available adsorbed coatings. This new adsorbed coating is highly stable and easy to produce in quantity, making it quite unique, and further making it possible to operate with any DNA sieving matrix. Finally, the above‐mentioned properties facilitate coating regeneration by a simple wash with a strongly alkaline solution, thus extending the lifetime of the capillary, a highly desirable property for any coating used in biopolymer separations.