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Gel electrophoretic mobility of single‐stranded DNA: The two reptation field‐dependent factors
Author(s) -
Rousseau Jean,
Drouin Guy,
Slater Gary W.
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000501)21:8<1464::aid-elps1464>3.0.co;2-e
Subject(s) - reptation , chemical physics , electrophoresis , field (mathematics) , chemistry , dna , polymer , statistical physics , physics , chromatography , mathematics , organic chemistry , pure mathematics , biochemistry
The reptation model is the dominant theory in understanding the electrophoretic separation of single‐stranded DNA molecules in gels or entangled polymer solutions. Recently, we showed that the Ogston and reptation regimes are separated by an entropic trapping regime at low field intensities. Here, we report the first comparison of the field‐dependent part of the DNA mobility for both small and long reptating molecules. We show that both mobilities increase linearly with field intensity, with the mobility of the longer (comigrating) fragments increasing faster than that of the smaller ones. We compare our results to the predictions of the biased reptation model.