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Fish muscle parvalbumins as marker proteins for native and urea isoelectric focusing
Author(s) -
Rehbein Hartmut,
Kündiger Rainer,
Pineiro Carmen,
PerezMartin Ricardo I.
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000501)21:8<1458::aid-elps1458>3.0.co;2-t
Subject(s) - isoelectric focusing , isoelectric point , urea , fish <actinopterygii> , chromatography , chemistry , muscle protein , biochemistry , biology , fishery , anatomy , enzyme , skeletal muscle
An isoelectric point (p I ) calibration kit containing fish muscle parvalbumins was prepared and tested for its suitability for isoelectric focusing (IEF) in the presence of 8 M urea. The pattern obtained by urea CleanGel IEF consisted of nine bands covering the p I range 4.96—5.64. This range is relevant for species identification of heated fish by urea IEF. The kit may also be used for native IEF in the low pH range, as demonstrated by running an extract made from the kit together with water‐soluble fish muscle proteins on Servalyt Precotes 3—6.