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Determination of clozapine by capillary zone electrophoresis following end‐column amperometric detection with simplified capillary/electrode alignment
Author(s) -
Jin Wenrui,
Xu Qiang,
Li Wei
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000401)21:7<1415::aid-elps1415>3.0.co;2-m
Subject(s) - capillary electrophoresis , amperometry , chromatography , capillary action , capillary electrochromatography , chemistry , electrode , analytical chemistry (journal) , materials science , electrochemistry , composite material
Capillary zone electrophoresis was employed for the determination of clozapine using an end‐column amperometric detection at a carbon fiber array microdisk electrode with simplified capillary/electrode alignment. The optimum conditions of separation and detection are: Britton‐Robinson buffer, pH 2.0 (1.3 × 10 —2 mol/L total concentration of acids, 3.2 × 10 —3 mol/L NaOH), 15 kV for separation voltage, 5 kV and 10 s for injection voltage and injection time, respectively. The limit of detection is 4.2 × 10 —7 mol/L or 1.2 fmole (signal to noise, S/N = 2). The relative standard deviation is 1.4% for the migration time and 2.5% for the electrophoretic peak current. The method was applied to the determination of clozapine in human blood. The recovery of the method is between 94—104%.