Premium
Quantitative assay of metronidazole by capillary zone electrophoresis with amperometric detection at a gold microelectrode
Author(s) -
Jin Wenrui,
Li Wei,
Xu Qiang,
Dong Qian
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000401)21:7<1409::aid-elps1409>3.0.co;2-0
Subject(s) - capillary electrophoresis , amperometry , detection limit , chromatography , chemistry , microelectrode , analytical chemistry (journal) , electrophoresis , electrode , electrochemistry
Capillary zone electrophoresis was employed for the determination of metronidazole using end‐column amperometric detection with a gold microelectrode at a constant potential of −0.52V vs. saturated calomel electrode. To overcome interference of oxygen in the solution, a deaeration injector and a deaeration protector at the detection cell were used. The optimum conditions of separation and detection are 1.0 × 10 −3 mol/L potassium dihydrogen citrate (KH 2 C 6 H 5 O 7 ) for the buffer solution, 20 kV for the separation voltage, and 5 kV and 10 S for injection voltage and injection time, respectively. The limit of detection is 6.0 × 10 7 mol/L or 0.78 fmole (S/N = 3). The relative standard deviation is 3.9% for the electrophoretic peak current. The method was applied to the determination of metronidazole in human urine.