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Evaluation of the potentiometric detection of acetylcholine and other neurotransmitters in capillary electrophoresis
Author(s) -
Kappes Thomas,
Schnierle Peter,
Hauser Peter C.
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000401)21:7<1390::aid-elps1390>3.0.co;2-5
Subject(s) - amperometry , detection limit , potentiometric titration , capillary electrophoresis , chromatography , chemistry , electrophoresis , acetylcholine , potentiometric sensor , electrode , analytical chemistry (journal) , electrochemistry , pharmacology , medicine
It is demonstrated that acetylcholine may be determined potentiometrically in capillary electrophoresis down to 6 × 10 —7 M with a miniature coated‐wire ion selective electrode. The more established amperometric method is not possible for this compound. Similarly, 4‐aminobutyric acid (GABA) is not accessible amperometrically; although potentiometric detection was possible, the sensitivity is not good. Other neurotransmitters may be determined by either method, but in these cases amperometric determination is generally favorable. For serotonin the potentiometric detection limit was 4 × 10 —5 M , while for the amperometric method a detection limit of 10 —7 M was found.