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Construction and evaluation of a capillary array DNA sequencer based on a micromachined sheath‐flow cuvette
Author(s) -
Crabtree H. John,
Bay Sue J.,
Lewis Darren F.,
Zhang Jianzhong,
Coulson Larry D.,
Fitzpatrick Glen A.,
Delinger Scott L.,
Harrison D. Jed,
Dovichi Norman J.
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000401)21:7<1329::aid-elps1329>3.0.co;2-2
Subject(s) - cuvette , photodiode , transimpedance amplifier , capillary action , materials science , laser induced fluorescence , optics , laser , capillary electrophoresis , amplifier , optoelectronics , chemistry , chromatography , operational amplifier , physics , cmos , composite material
A capillary array electrophoresis DNA sequencer is reported based on a micromachined sheath‐flow cuvette as the detection chamber. This cuvette is equipped with a set of micromachined features that hold the capillaries in precise registration to ensure uniform spacing between the capillaries, in order to generate uniform hydrodynamic flow in the cuvette. A laser beam excites all of the samples simultaneously, and a microscope objective images fluorescence onto a set of avalanche photodiodes, which operate in the analog mode. A high‐gain transimpedance amplifier is used for each photodiode, providing high duty‐cycle detection of fluorescence.