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Fluorescence line‐narrowing detection in chromatography and electrophoresis
Author(s) -
Jankowiak Ryszard,
Roberts Kenneth P.,
Small Gerald J.
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000401)21:7<1251::aid-elps1251>3.0.co;2-d
Subject(s) - capillary electrophoresis , chromatography , capillary electrochromatography , fluorescence spectroscopy , chemistry , resolution (logic) , high performance liquid chromatography , fluorescence , analytical chemistry (journal) , electrochromatography , analyte , optics , artificial intelligence , computer science , physics
A review of the basic aspects of fluorescence line‐narrowing spectroscopy (FLNS) and its coupling with thin‐layer chromatography (TLC) and polyacrylamide gel electrophoresis (PAGE) for off‐line high‐resolution low temperature spectral characterization is discussed. This is followed by a description of the on‐line interfacing of capillary electrophoresis (CE) and capillary electrochromatography (CEC) with FLN detection. CE/CEC‐FLNS instrumentation and its applications for spectral identification of closely related analytes are also presented. Future prospects of micro and capillary high performance liquid chromatography (HPLC) with on‐line high‐resolution low temperature spectroscopic identification are considered.