Premium
Detection in capillary electrophoresis
Author(s) -
Swinney Kelly,
Bornhop Darryl J.
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000401)21:7<1239::aid-elps1239>3.0.co;2-6
Subject(s) - capillary electrophoresis , detection limit , chromatography , electrophoresis , chemistry , computer science
A review of the four major, on‐line, capillary electrophoresis (CE) detection modalities is presented. It is shown that each detection method, fluorescence, absorbance (conventional and nonconventional), electrochemical and refractive index, have distinct advantages and limitations when applied to analysis in a CE format. Various aspects of CE detection are considered and a perspective regarding the applicability of the technique is provided. It is shown that because of widely varying detection limits (ranging from single molecule to 10 —5 M ) and detection scheme complexity, the particular application should dictate the selection of detection methodology in CE.