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Recent advancements in surface‐enhanced laser desorption/ionization‐time of flight‐mass spectrometry
Author(s) -
Merchant Maggie,
Weinberger Scot R.
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000401)21:6<1164::aid-elps1164>3.0.co;2-0
Subject(s) - mass spectrometry , surface enhanced laser desorption/ionization , biomarker discovery , time of flight mass spectrometry , laser , biomarker , chemistry , desorption , proteomics , chromatography , ionization , protein mass spectrometry , electrospray ionization , adsorption , physics , optics , biochemistry , ion , organic chemistry , gene
The overall history and recent advances in surface enhanced laser desorption/ionization‐time of flight‐mass spectrometry (SELDI‐TOF‐MS) technology is reviewed herein. Fundamentals of SELDI‐TOF analysis are presented while drawing comparisons with other laser‐based mass spectrometry techniques. The application of SELDI‐TOF‐MS to functional genomics and biomarker discovery is discussed and exemplified by elucidating a biomarker candidate for prostatic carcinoma. Finally, a short discussion regarding future SELDI requirements and developments is supplied.