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Membrane proteins and proteomics: Un amour impossible?
Author(s) -
Santoni Véronique,
Molloy Mark,
Rabilloud Thierry
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000401)21:6<1054::aid-elps1054>3.0.co;2-8
Subject(s) - proteome , proteomics , membrane protein , gel electrophoresis , electrophoresis , two dimensional gel electrophoresis , computational biology , biology , membrane , chemistry , biochemistry , gene
Proteome analysis implies the ability to separate proteins as a first step prior to characterization. Thus, the overall performance of the analysis strongly depends on the performance of the separation tool, usually two‐dimensional electrophoresis. This review shows how two‐dimensional electrophoresis performs with membrane proteins from bacteria or animal or vegetable cells and tissues, the recent progress in this field, and it examines future prospects in this area.