Premium
A new method of quantification of pipemidic acid by capillary zone electrophoresis with end‐column amperometric detection
Author(s) -
Jin Wenrui,
Yu Daiqing,
Dong Qian,
Ye Xiaoying
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000301)21:5<925::aid-elps925>3.0.co;2-t
Subject(s) - capillary electrophoresis , amperometry , detection limit , chromatography , saturated calomel electrode , chemistry , electrophoresis , analytical chemistry (journal) , capillary action , electrode , working electrode , materials science , electrochemistry , composite material
Capillary zone electrophoresis was employed for the determination of pipemidic acid using an end‐column amperometric detection with a carbon fiber microdisk array electrode, at a constant potential of −1.10 V vs. saturated calomel electrode. The optimum conditions of separation and detection were 1.2 × 10 −4 mol/LNaOAc — 8.8 × 10 −4 mol/LHOAc for the buffer solution, 20 kV for the separation voltage, 5 kV and 10 s for the injection voltage and the injection time. The limit of detection was 1.05 × 10 −6 mol/L or 189 amol (S/N=3). The relative standard deviation was 0.31% for the migration time and 2.0% for the electrophoretic peak current. The method was applied to determining pipemidic acid in human serum.