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New adsorbed coatings for capillary electrophoresis
Author(s) -
Chiari Marcella,
Cretich Marina,
Damin Francesco,
Ceriotti Laura,
Consonni Roberto
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000301)21:5<909::aid-elps909>3.0.co;2-l
Subject(s) - adsorption , capillary electrophoresis , polymer , capillary action , coating , chemistry , electrophoresis , chemical engineering , chromatography , materials science , organic chemistry , composite material , engineering
New acrylic polymers bearing oxirane groups were synthesized to be used in the production of coated capillaries. A fully automated coating procedure was devised based on the use of diluted water solutions of these polymers. The whole procedure required less than 30 min. The new polymers rapidly adsorbed from water onto the capillary wall, thus suppressing electroosmotic flow (EOF) to a negligible value. The adsorbed coatings were stable for hundreds of hours at high pH, temperature, and in the presence of 8 M urea. Efficient separations of acidic and basic proteins were achieved in the new phases.