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On‐line identification of depurinating DNA adducts in human urine by capillary electrophoresis — fluorescence line narrowing spectroscopy
Author(s) -
Roberts Kenneth P.,
Lin ChengH.,
Singhal Maria,
Casale George P.,
Small Gerald J.,
Jankowiak Ryszard
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000301)21:4<799::aid-elps799>3.0.co;2-v
Subject(s) - capillary electrophoresis , urine , adduct , chemistry , chromatography , fluorescence spectroscopy , pyrene , guanine , fluorescence , dna , biochemistry , organic chemistry , gene , nucleotide , physics , quantum mechanics
The benzo[ a ]pyrene (BP)‐derived 7‐(benzo[ a ]pyren‐6‐yl)guanine (BP‐6‐N7Gua) depurinating one‐electron oxidation adduct was identified in the urine extracts of coal‐smoke‐exposed humans for the first time. Urine samples were prepared by solid‐phase extraction and reversed‐phase high‐performance liquid chromatography. Subsequently, the BP‐6‐N7Gua adduct was identified on‐line with capillary electrophoresis ‐ fluorescence line narrowing spectroscopy (CE‐FLNS) at 4.2 K. The daily excretion of BP‐6‐N7Gua in human urine of individuals exposed to coal smoke was approximately 226 pmol per μmol of creatinine. Due to the high level of excretion we propose that BP‐6‐N7Gua adducts found in urine could serve as effective biomarkers for risk assessment of BP exposure. The results demonstrate that CE‐FLNS allows for on‐line separation and DNA adducts identification in complex fluid extracts.