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Preparative‐scale isoelectric focusing separation of enantiomers using a multicompartment electrolyzer with isoelectric membranes
Author(s) -
Glukhovskiy Pavel,
Landers Terry A.,
Vigh Gyula
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000301)21:4<762::aid-elps762>3.0.co;2-y
Subject(s) - isoelectric focusing , chromatography , membrane , enantiomer , chemistry , isoelectric point , cyclodextrin , electrolysis , electrophoresis , electrolyte , organic chemistry , enzyme , biochemistry , electrode
The IsoPrime multicompartment electrolyzer, equipped with a series of isoelectric membranes with closely spaced p I values, was used for the first time for the preparative‐scale separation of the enantiomers of dansyl phenylalanine with hydroxypropyl β‐cyclodextrin as resolving agent. The final separation conditions could be established easily in three successive experiments by rationally narrowing the pH steps between the neighboring isoelectric membranes. The final separation yielded products with an enantiomeric excess greater than 99.9%, at production rates of about 0.1 mg/h. The greatest experimental difficulty was caused by the relatively high salt content of the hydroxypropyl β‐cyclodextrin used, which resulted in high conductivity and limited the maximum field strength one could use.