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Measurement of bilirubin partition coefficients in bile salt micelle/aqueous buffer solutions by micellar electrokinetic chromatography
Author(s) -
Maeder Corina,
Beaudoin Gerard M. J.,
Hsu Ekai,
Escobar Veronica A.,
Chambers Stuart M.,
Kurtin William E.,
Bushey Michelle M.
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000301)21:4<706::aid-elps706>3.0.co;2-5
Subject(s) - micelle , chemistry , partition coefficient , chromatography , critical micelle concentration , micellar electrokinetic chromatography , aqueous solution , capacity factor , salt (chemistry) , taurocholic acid , buffer solution , analytical chemistry (journal) , bile acid , detection limit , biochemistry , high performance liquid chromatography , organic chemistry
The partition coefficients for the distribution of bilirubin between aqueous phosphate‐borate buffer and cholic, taurocholic, taurodeoxycholic, and taurochenodeoxycholic micelles have been measured by micellar electrokinetic chromatography at pH 8.5. Determination of the partition coefficients required that the critical micelle concentration and partial specific volumes be determined for each bile salt. Critical micelle concentrations were slightly higher for the trihydroxy bile salts. Partial specific volumes of the bile salt micelles differed very little from each other, and for each bile salt they were constant over the concentration range studied, which was typically from slightly above the critical micelle concentration to 35 m M . Capacity factors were corrected for the effects of applied voltage by extrapolation of the capacity factor to zero applied volts. The free solution mobility of bilirubin, determined in the absence of bile salt, was also corrected for the effects of applied voltage. Plots of extrapolated capacity factor versus phase ratio yield the partition coefficient as the slope of a linear fit to the data. Partition coefficients for bilirubin were significantly higher for dihydroxy bile salts than for trihydroxy bile salts.