Premium
Protein patterns of gel‐entrapped Escherichia coli cells differ from those of free‐floating organisms
Author(s) -
Perrot Frederique,
Hébraud Michel,
Charlionet Roland,
Junter GuyAlain,
Jouenne Thierry
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000201)21:3<645::aid-elps645>3.0.co;2-1
Subject(s) - escherichia coli , bacteria , biochemistry , stationary phase , gel electrophoresis , electrophoresis , chemistry , biology , chromatography , gene , genetics
The two‐dimensional electrophoretic patterns of cellular proteins from gel‐entrapped Escherichia coli cells were compared to those of exponential‐ and stationary‐phase free‐floating organisms. The amounts of several proteins in immobilized cells were significantly different from those in free bacteria. Immobilized organisms rapidly produced a high level of dipeptide permease and a single‐strand binding protein, and progressively accumulated an aldehyde dehydrogenase. Immobilization also induced a decrease in the levels of two proteins, i.e. , the YFID protein and a DNA‐binding, stationary‐phase protein. The possible role of these proteins in the high resistance of immobilized bacteria to stresses is discussed.