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New carrier electrolytes for the separation of chlorophenols by capillary electrophoresis
Author(s) -
Jáuregui Olga,
Puignou Lluís,
Galceran M. Teresa
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000201)21:3<611::aid-elps611>3.0.co;2-t
Subject(s) - capillary electrophoresis , electrolyte , chemistry , chromatography , electrophoresis , ammonium acetate , capillary action , electrochromatography , coating , capillary electrochromatography , analytical chemistry (journal) , electrode , high performance liquid chromatography , materials science , organic chemistry , composite material
Optimum conditions for the separation of positional isomers of chlorophenols by capillary zone electrophoresis (CZE) were established. The behavior of five volatile electrolytes ( L ‐cysteic acid, 3‐amino‐1‐propanesulfonic acid, aminomethanesulfonic acid, diethylmalonic acid, and ammonium acetate) was compared. The best performance based on low electrophoretic current and high separation efficiency was obtained for diethylmalonic acid as working electrolyte. The influence of pH on the separation, using both uncoated fused‐silica capillaries and modified capillaries (NaAMPS from EKT) with anionic coating, was discussed. Moreover, the effect of electrolyte concentration and applied voltage using fused‐silica capillaries was studied. The optimum CZE conditions that allowed the separation of 16 chlorophenols were 20 kV, 30 m M diethylmalonic acid, pH 7.25, and uncoated fused‐silica capillary. Figures of merit such as run‐to‐run and day‐to‐day precision, linearity, and limits of detection were calculated.