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Automatic calibration in capillary electrophoresis
Author(s) -
Arce Lourdes,
Hinsmann Peter,
Novic Milko,
Ríos Angel,
Valcárcel Miguel
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000201)21:3<556::aid-elps556>3.0.co;2-y
Subject(s) - capillary electrophoresis , calibration , analyte , peristaltic pump , microprocessor , computer science , chromatography , capillary action , electrophoresis , calibration curve , computer hardware , analytical chemistry (journal) , chemistry , materials science , detection limit , engineering , mathematics , mechanical engineering , statistics , composite material
A direct, completely automated calibration procedure in capillary electrophoresis (CE) is presented. The manual calibration operations involved in analytical methods, such as external calibration, standard addition, and internal standard, were accomplished with a continuous flow system (CFS) coupled to commercial capillary electrophoresis equipment via a programmable arm. The system was managed by the proper CE microprocessor and allowed automatic calibration and direct quantification of the analytes in the sample without any manual pretreatment, therefore avoiding or minimizing manually associated errors. The whole system including peristaltic pumps and the programmable arm was connected via an electronic interface and completely controlled by a computer using a program written in GW‐BASIC. The potential of this new CFS‐CE arrangement was assessed by applying it to samples containing selected compounds.