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A method to produce Ponceau replicas from blots: Application for Western analysis
Author(s) -
Gotzmann Josef,
Gerner Christopher
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000201)21:3<523::aid-elps523>3.0.co;2-l
Subject(s) - blot , simple (philosophy) , chemistry , computer science , chromatography , biochemistry , gene , philosophy , epistemology
We describe a fast and simple method to produce print‐quality like Ponceau replicas from blots. These have many applications for Western analysis, primarly to alleviate localization of antigens in complex protein patterns generated by two‐dimensional electrophoresis.