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Electropherogram of capillary zone electrophoresis with effective mobility axis as a transverse axis and its analytical utility I. Transformation applying the hypothetical electroosmotic flow
Author(s) -
Ikuta Natsuki,
Yamada Yuji,
Hirokawa Takeshi
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000101)21:2<360::aid-elps360>3.0.co;2-t
Subject(s) - electropherogram , capillary electrophoresis , transformation (genetics) , joule heating , electrophoresis , capillary action , potential gradient , isotachophoresis , electro osmosis , chemistry , transverse plane , flow (mathematics) , analytical chemistry (journal) , voltage , materials science , mechanics , chromatography , physics , electrode , electrolyte , composite material , biochemistry , structural engineering , quantum mechanics , engineering , gene
For capillary zone electrophoresis, a new method of transformation from migration time to effective mobility was proposed, in which the mobility increase due to Joule heating and the relaxation effect of the potential gradient were eliminated successfully. The precision of the mobility evaluated by the proposed transformation was discussed in relation to the analysis of rare earth ions. By using the transformation, almost the same pherograms could be obtained even from the pherograms obtained originally at different applied voltages.