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Short tandem repeat analysis in Japanese population
Author(s) -
Hashiyada Masaki
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000101)21:2<347::aid-elps347>3.0.co;2-9
Subject(s) - microsatellite , polymerase chain reaction , str multiplex system , dna extraction , biology , multiplex polymerase chain reaction , str analysis , multiplex , genomic dna , dna sequencer , population , genetics , microbiology and biotechnology , dna , allele , gene , medicine , environmental health
Short tandem repeats (STRs), known as microsatellites, are one of the most informative genetic markers for characterizing biological materials. Because of the relatively small size of STR alleles (generally 100—350 nucleotides), amplification by polymerase chain reaction (PCR) is relatively easy, affording a high sensitivity of detection. In addition, STR loci can be amplified simultaneously in a multiplex PCR. Thus, substantial information can be obtained in a single analysis with the benefits of using less template DNA, reducing labor, and reducing the contamination. We investigated 14 STR loci in a Japanese population living in Sendai by three multiplex PCR kits, GenePrint™ PowerPlex™ 1.1 and 2.2. Fluorescent STR System (Promega, Madison, WI, USA) and AmpF I STR Profiler™ (Perkin‐Elmer, Norwalk, CT, USA). Genomic DNA was extracted using sodium dodecyl sulfate (SDS) proteinase K or Chelex 100 treatment followed by the phenol/chloroform extraction. PCR was performed according to the manufacturer's protocols. Electrophoresis was carried out on an ABI 377 sequencer and the alleles were determined by GeneScan™ 2.0.2 software (Perkin‐Elmer). In 14 STRs loci, statistical parameters indicated a relatively high rate, and no significant deviation from Hardy‐Weinberg equilibrium was detected. We apply this STR system to paternity testing and forensic casework, e.g. , personal identification in rape cases. This system is an effective tool in the forensic sciences to obtain information on individual identification.