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Heat shock protein (hsp 70)‐related epitopes are common allergenic determinants for barley and corn antigens
Author(s) -
Chiung YinMei,
Lin BaiLing,
Yeh ChinHui,
Lin ChuYung
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000101)21:2<297::aid-elps297>3.0.co;2-u
Subject(s) - heat shock protein , gel electrophoresis , polyacrylamide gel electrophoresis , sodium dodecyl sulfate , molecular mass , epitope , chemistry , allergen , pulsed field gel electrophoresis , antigen , hsp60 , microbiology and biotechnology , biology , biochemistry , hsp70 , immunology , gene , genotype , enzyme , allergy
IgE reactive components of barley and corn were compared using sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and immunoblotted with sera from workers exposed to complex bioaerosols. The antibody made against Arabdopsis heat shock protein (hsp 70) was used to identify those components equivalent to hsp 70 in molecular size. Components with a molecular mass of 69 kDa and 33 kDa were positively reacted, and immunoblots of two‐dimensional polyacrylamide gel electrophoresis were compared.