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High‐speed chiral separations on microchip electrophoresis devices
Author(s) -
Rodríguez Isabel,
Ji Jin Lian,
Li Sam F. Y.
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(20000101)21:1<211::aid-elps211>3.0.co;2-d
Subject(s) - micellar electrokinetic chromatography , enantiomer , chromatography , electrokinetic phenomena , electrophoresis , chemistry , resolution (logic) , cyclodextrin , buffer (optical fiber) , capillary electrophoresis , amino acid , analytical chemistry (journal) , stereochemistry , telecommunications , biochemistry , artificial intelligence , computer science
High‐speed electrophoretic chiral separations have been successfully performed in a microfabricated device by employing cyclodextrin‐modified micellar electrokinetic chromatography (CD‐MEKC). Utilizing short separation channels and relatively high field strengths in combination with small volume‐defined injection plugs, and operating in counter‐electroosmotic flow conditions, fast and efficient separations of fluorescein insothiocyanate (FITC)‐labeled amino acid enantiomers were obtained. Analysis time ranged from 75 s for the most basic amino acids to 160 s for the most acidic ones with associated efficiencies from 7000 up to 28 000 effective plates (100 000 to 395 000 plates/m). Buffer parameters were varied in order to study the effect on chiral resolution. A buffer system consisting of 100 m M borate (pH 9.4), 30 m M of SDS, and 10 m M γ‐CD as chiral selector provided adequate resolution of the majority of FITC‐amino acid enantiomers tested.