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Rat brain proteins: Two‐dimensional protein database and variations in the expression level
Author(s) -
Fountoulakis Michael,
Schuller Elisabeth,
Hardmeier Rosemarie,
Berndt Peter,
Lubec Gert
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19991201)20:18<3572::aid-elps3572>3.0.co;2-t
Subject(s) - heat shock protein , proteome , proteomics , gel electrophoresis , biology , mass spectrometry , enzyme , protein expression , two dimensional gel electrophoresis , database , chemistry , computational biology , biochemistry , chromatography , gene , computer science
A two—dimensional database of rat brain proteins was constructed. Brain samples from newborn animals were analyzed by two—dimensional electrophoresis and the proteins were identified by matrix‐assisted laser desorption/ionization mass spectrometry. The dababase comprises 210 different proteins, the majority of which are structural components, heat shock proteins and enzymes with various catalytic activities. Several minor differences in the expression level were detected, mainly of quantitative nature, which most likely represent allelic differences. The map may be useful in studies of neurological disorders in animal models of human diseases.