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Determination of free calcium in guinea‐pig cochlea perilymph by capillary electrophoresis with direct injection
Author(s) -
Guo Weiying,
Zhang Yongmei,
Chen Dong,
Bai Xiuzhen,
Zhao Wei,
Ma Yong
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19991101)20:17<3455::aid-elps3455>3.0.co;2-n
Subject(s) - perilymph , guinea pig , cochlea , calcium , capillary electrophoresis , chemistry , chromatography , biophysics , anatomy , endocrinology , medicine , biology , organic chemistry
At present, the tinnitus mechanism is still not clear. Our experiments demonstrated that the concentration of free calcium in cochlea perilymph of tinnitus model guinea‐pigs is lower than that in normal guinea‐pigs. However, the volume of conchlea perilymph is so small that only 5—10 μL of sample can be obtained from each animal. We describe the application of CE to the detectionl of free calcium in guinea‐pig cochlea perilymph. Direct injection was employed in this study. The separation was carried out at 10 kV. The capillary temperature was maintained at 20°C, and indirect UV detection at 214 nm was employed. The samples were vacuum injected for 3 s. The run buffer was 0.005 mol/L imidazole with a pH of 4.30—4.50. The concentration of free calcium in the normal group was found to be in accordance with the reference data. The method has been applied to research on the tinnitus mechanism and for medical treatment.