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Capillary electrophoresis‐diode array detection — electrospray mass spectrometry for the analysis of selected tropane alkaloids in plant extracts
Author(s) -
Mateus Lidia,
Cherkaoui Samir,
Christen Philippe,
Veuthey JeanLuc
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19991101)20:17<3402::aid-elps3402>3.0.co;2-e
Subject(s) - hyoscyamine , capillary electrophoresis , chromatography , chemistry , electrospray , mass spectrometry , tropane , electrospray ionization , capillary electrophoresis–mass spectrometry , datura stramonium , datura , alkaloid , analytical chemistry (journal) , solanaceae , organic chemistry , botany , biochemistry , biology , gene
Capillary zone electrophoresis, coupled to UV and interfaced with electrospray ionization mass spectrometry (ESI‐MS), is described for the simultaneous analysis of hyoscyamine and scopolamine. On‐line UV detection occurred at 22 cm from the inlet of the capillary and ESI‐MS monitoring was performed along the entire length of the capillary (85 cm). An alkaline solution of 40 m M ammonium acetate at pH 8.5 was suitable for the analysis of the alkaloids under consideration. Under the optimized conditions, including CE and ESI‐MS parameters, the two alkaloids were resolved within a short time and with very high sensitivity. The differentiation of hyoscyamine and its positional isomer littorine, commonly encountered in plant material, is also presented using up‐front collision‐induced dissociation. Finally, the developed method was applied to the analysis of these alkaloids in Belladonna leaf extract and in Datura candida x D. aurea hairy root extract.