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Capillary electrophoresis in sieving matrices: Selectivity per base, mobility slope, and inflection slope
Author(s) -
Dolník Vladislav,
Gurske William A.
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19991101)20:17<3373::aid-elps3373>3.0.co;2-0
Subject(s) - inflection point , reptation , capillary electrophoresis , matrix (chemical analysis) , chemistry , materials science , analytical chemistry (journal) , chromatography , mathematics , geometry , polymer , organic chemistry
We compare the migration behavior of DNA sequencing fragments in hydroxyethyl cellulose (HEC) to the theoretical model of migration in the reptation mode. Good agreement was found for the mobility curve. We derived empirical equations for the relationship between selectivity per base and sieving matrix concentration and between the mobility slope and matrix concentration. We propose the inflection slope, i.e. , the slope of the log‐log mobility curve at its inflection point, as the quantitative parameter of sieving performance.