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Electrophoretic and serologic characterization of 56 kDa antigen (M56) with autologous serum derived from a chondrosarcoma patient: A shared antigen of immunoresponses in cancer and autoimmune diseases
Author(s) -
Fujiwara Kazuo,
Udono Heiichiro,
Kunisada Toshiyuki,
Kawai Akira,
Inoue Hajime,
Takigawa Masaharu,
Namba Masayoshi,
Nakayama Eiichi
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19991101)20:17<3335::aid-elps3335>3.0.co;2-x
Subject(s) - antibody , antigen , immune system , immunology , chondrosarcoma , cancer , gel electrophoresis , microbiology and biotechnology , medicine , biology , pathology
We investigated whether antibodies specific to autologous cancer cells are produced in the peripheral blood of patients with chondrosarcoma. There have been few reports on the investigation of the immune responses, such as autologous antibody production, to chondrosarcoma. Here, tumor‐associated antigens were separated by sodium dodecyl sulfate (SDS)‐polyacrylamide gel electrophoresis and detected by immuno‐enzymatic amplification. A 56 kDa molecule (M56) was detected in the serum from patients' peripheral blood. M56 is ubiquitously expressed in various kinds of tissue‐derived cells. However, the molecule seemed to be retained mostly in the cytosolic compartment of lymphoid cells, while it was expressed on the cell surface of nonlymphoid cancer cells. Furthermore, the antibodies reactive to the 56 kDa molecule were frequently observed in sera derived from patients with other cancers and autoimmune diseases as compared to the sera from healthy control donors, suggesting that M56 is a common target molecule of immune responses in patients with various cancers and autoimmune diseases.