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Applications of capillary electrophoresis in biotechnology
Author(s) -
Lagu Avinash L.
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19991001)20:15/16<3145::aid-elps3145>3.0.co;2-0
Subject(s) - oligonucleotide , capillary electrophoresis , recombinant dna , escherichia coli , dna , biochemistry , electrophoresis , gel electrophoresis , biology , computational biology , chromatography , chemistry , microbiology and biotechnology , gene
Capillary electrophoresis (CE)‐related techniques are increasingly being used as a matter of routine practice in the biotechnology discipline. Since recombinant DNA‐derived proteins and the antisense oligonucleotides constitute a large portion of the applications of these techniques, they have been emphasized in this review. Analyses by CE of Escherichia coli ‐derived proteins and glycosylated proteins derived from mammalian cell cultures are summarized, as well as those of the carbohydrate chains that have been enzymatically removed from the protein. Applications of CE in the analysis of the antisense oligonucleotides for the determination of purity and the analytical studies on the metabolism of these modified oligonucleotides, by CE are reviewed. The literature mainly covers the period from 1996.